Phytoalexin Accumulation in Sorghum

The Accumulation of Phytoalexins in Resistant Sorghum Leaves.

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Fig. 1

(a) Surface view of a sorghum leaf inoculated 24 hours previously with C. sublineolum. Colourless inclusions accumulate in the cytoplasm of cells beneath fungal appressoria.

(b) By 30 hours after inoculation, inclusions have increased in size and become pigmented orange.

 Fig. 2
Fig. 3

(c) As time progresses, the inclusions become more intensely red pigmented, and start to accumulate in greater numbers around the site of appressorium attachment.

(d) By 36 hours after inoculation, the inclusion have started to coalesce and are intensely red pigmented.

 Fig. 4
Fig. 5

(e) By 42 hours after inoculation, the inclusions have released their contents into the cell and pigmented inclusions are starting to accumulate in surrounding cells.

(f) By 54 hours after inoculation, the inclusions in the surrounding cells have released their contents into the cells and the cell walls are intensely stained red.

 Fig. 6

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